FISH and chips: Automation of fluorescent dot counting in interphase cell nuclei
نویسندگان
چکیده
منابع مشابه
FISH and chips: automation of fluorescent dot counting in interphase cell nuclei.
Fluorescence in situ hybridization allows the enumeration of chromosomal abnormalities in interphase cell nuclei. This process is called dot counting. To estimate the distribution of chromosomes per cell, a large number of cells have to be analyzed, especially when the frequency of aberrant cells is low. Automation of dot counting is required because manual counting is tedious, fatiguing, and t...
متن کاملFluorescent dot counting in interphase cell nuclei
Fluorescence in situ hybridization allows the enumeration of chromosomal abnormalities in interphase cell nuclei. This process is called dot counting. To estimate the distribution of chromosomes per cell, a large number of cells have to be analysed, particularly when the frequency of aberrant cells is low. Automation of dot counting is desirable because manual counting is tedious, fatiguing, an...
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We have developed a completely automated fluorescence microscope system that can examine 500 cells in approximately 20 minutes to determine the number of labeled chromosomes (seen as dots) in each cell nucleus. This system works with two fluorescent dyes – one for the DNA hybridization dots (e.g. FITC) and one for the cell nucleus (e.g. DAPI). After the stage has moved to a new field the image ...
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The evaluation of an automated system for Fluorescence In Situ Hybridization (FISH) spot counting in interphase nuclei is presented in this paper. Different types of experiments have been performed with samples from known populations. In all of them the goal is to detect mosaicism of chromosome X in leukocytes from mixtures in known proportions of healthy male and female blood. First the initia...
متن کاملTowards many colors in FISH on 3D-preserved interphase nuclei.
The article reviews the existing methods of multicolor FISH on nuclear targets, first of all, interphase chromosomes. FISH proper and image acquisition are considered as two related components of a single process. We discuss (1) M-FISH (combinatorial labeling + deconvolution + wide-field microscopy); (2) multicolor labeling + SIM (structured illumination microscopy); (3) the standard approach t...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1997
ISSN: 0196-4763,1097-0320
DOI: 10.1002/(sici)1097-0320(19970501)28:1<1::aid-cyto1>3.3.co;2-n